文献综述
基于数字微镜阵列的全场 OCT 结构光场设计
摘要：
全场光学相干层析术是一种非侵入无标记的高分辨生物组织成像技术，它结合低相干光源的白光干涉技术和显微物镜，以及面阵成像相机，对生物组织进行二维层析成像。FF-OCT运用低相干光波，利用单次后向散射光保留光源相干性的特点，通过低相干光光源的相干门把其他散射光排除在外，从而获得微米量级的生物样品内部信息。结合纵向扫描成像方式，可实现对生物组织三维结构微米量级的层析成像。结构照明系统通常选择旋转光栅和空间光调制器实现结构照明的实施方案。但采用机械的方式来控制光栅的旋转和位移，其装置复杂，旋转和位移的速度较低，不同激发波长对应plusmn;1级衍射角不一样，波长改变时需要调节光路。液晶空间光调制器虽然可以提高产生和控制结构条纹的速度和精度，但只能对偏振光进行调制。因此，我们将用DMD数字微镜阵列代替传统的结构照明体统，本文主要整理了结构照明超分辨成像的发展与研究现状，全场光学相干层析术的相关原理以及DMD数字阵列的主要应用，为下一步的研究打好理论基础，希望通过本次整理对超分辨显微成像技术的发展有积极进步作用。
关键词：超分辨显微成像；数字微镜阵列（DMD）；全场光学相干层析术（FF-OCT）
Abstract:
Full-field optical coherence tomography is a non-invasive and unmarked high-resolution imaging technique for biological tissues. It combines the white light interference technique with low coherence light source, microscopic objective lens and planar array imaging camera to perform two-dimensional tomography for biological tissues. Ff-oct uses low-coherence light waves, uses single-order backscatter light to retain the coherence of the light source, and excludes other scattered light through the coherence door of the low-coherence light source, thus obtaining the internal information of biological samples in the micron scale. Combined with the longitudinal scan imaging method, the three-dimensional structure of biological tissue in micron scale tomography can be realized. In structural lighting systems, rotating gratings and spatial light modulators are usually selected to realize the implementation of structural lighting. However, the rotation and displacement of the grating are controlled mechanically. The device is complex and the speed of rotation and displacement is low. Different excitation wavelengths correspond to different diffraction angles of plusmn;1 order, and the optical path needs to be adjusted when the wavelength changes. Although liquid crystal spatial light modulator can improve the speed and precision of producing and controlling structural fringes, it can only modulate polarized light. Therefore, we will use the DMD digital micromirror array instead of the traditional lighting system structure, this article mainly organized structure lighting development and research status of super resolution imaging, full of relevant principle of the optical coherence tomography technique and main application of the DMD digital array, provided a theoretical basis for further study, hope that through this sort of super resolution microscopic imaging technology development has a positive progress.
Keywords: super-resolution microscopy; digital micromirror array (DMD); full-field optical coherence tomography (FF-OCT)
一、引言
